I set out to find the best way to collect bloodfed mosquitoes 🩸
Why collect bloodfed mosquitoes?
We use bloodfed mosquitoes to observe mosquito feeding patterns. For example, do they feed on birds or mammals?
And crucially . . . do they feed on humans?
A mosquito that feeds on both wild animals and humans could transmit a virus from the infected wild host to us!!
Therefore, each bloodfed mosquito is crucial. They provide direct evidence of a vector-host interaction and help us piece together the transmission cycles of mosquito borne diseases.
Why is it so hard to collect bloodfed mosquitoes?
Mosquitoes with a belly full of blood don’t fly into traditional light traps. Dang!
Instead they look for a quite place to rest and digest their bloodmeal, such as a tree hallow, tall grass, or small hole. These locations are hard to access. The bloodfed mosquitoes could be anywhere!
Then how do we collect bloodfeds?
In my research, I tested several methods to see which one is the most effective.
a) Direct aspiration of vegetation
I built a homemade aspirator and would use this to suck up mosquitoes from vegetation. I had the most success in humid, dense, tall vegetation growing in damp soil or on the edge of ponds.
Watch how it’s done! 🎥
b) I also created artifical resting shelters.
In the hope that bloodfed mosquitoes would choose to rest inside these shelters.
I tested large rubbish bins and felt bags hung from trees.
I compared how well these artificial resting shelters performed against aspiration.